Amptk

Latest version: v1.6.0

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1.2.3

* fix auto-detect of base name output files in clustering scripts
* bump version
* update citation as now published in PeerJ https://peerj.com/articles/4925/

1.2.2

* fix tab indentation bug for py3
* add minimum length and trimming length to log file and terminal output.

1.2.1

* numerous bug fixes: including a fix for `--require_primer off` in `amptk illumina`. Several bug fixes in `amptk illumina2` and `amptk illumina3` pre-processing steps.
* thread (processes) control added for clustering steps

1.2.0

* for all Illumina pre-processing methods the order of steps has been changed to now 1) search for primers/trim and then 2) merged PE reads. This ensures that all primer/adapter sequences are trimmed from dataset, whereas previously AMPtk releases merged PE reads first - because of how usearch/vsearch merge PE reads, occasional primer/adapter sequences were slipping through the pre-processing steps. Also reads with multiple primer hits (i.e. two forward primers) are now discarded. While this results in the pre-processing steps being slightly slower in runtime, it increases the data quality downstream.
* read orientation is tested/fixed on the fly for `amptk illumina2` workflow (barcodes/primers in reads). Some datasets of 50/50 read orientation.
* added a check for "inverted OTUs" for all denoising/clustering steps. This was largely a check to validate that changing illumina pre-processing steps were working correctly (as unintended result was a small number of OTUs that were on the "crick" strand)
* Default mapping file now has a 'RevBarcodeSequence' column. Mapping files used for `amptk illumina2` enforce the paired barcode sequences in the mapping file. If barcode fasta files are given, then all combinations of barcodes (5' and 3') are saved.
* a few py27/36 bug fixes

1.1.3

* update to `amptk taxonomy` to alert user if sample names are duplicated
* many fixes to unify the 'base name" output for many scripts
* remove colored date/time stamp for all platforms except Mac
* many fixes for py2/py3 compatibility
* `amptk` now moved to `bin` directory - will slightly change install

1.1.2

* update compatibility for py2/3

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