* Species classification is now based strictly on the ANI to reference genomes
* The "classify" function now reports the closest reference genome in the summary file even if the ANI is <95%
* The summary.tsv file has 4 new columns: aa_percent, red_values, fastani_reference_radius, and warnings
* By default, the "align" function now performs the same MSA trimming used by the GTDB
* New pplacer support for writing to a scratch file (--mmap-file option)
* Random seed option for MSA trimming has been added to allow for reproducible results
* Configuration of the data directory is now set using the environmental variable GTDBTK_DATA_PATH (see [pip installation](pip-installation))
* Perl dependencies has been removed
* Python libraries biolib, mpld3 and jinja have been removed
* This version requires a new version of the GTDB-Tk data package (gtdbtk.r86_v2_data.tar.gz) available [here](https://data.ace.uq.edu.au/public/gtdbtk/release_86/)